3/22/2023 0 Comments Pixel picker tool salsaj 2.3![]() ![]() These hallmark features were introduced by series editor Dr. Each protocol is provided in readily-reproducible step-bystep fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, UKįor over 35 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. Pages 465-467 Citation previewĬomputer Optimized Microscopy Methods and Protocols Machine Learning: Advanced Image Segmentation Using ilastik (Anna Kreshuk, Chong Zhang).Pages 449-463īack Matter. ![]() Butzin, Javier Buceta).Pages 399-422ĢD + Time Object Tracking Using Fiji and ilastik (Andrea Urru, Miguel Angel González Ballester, Chong Zhang).Pages 423-448 Moreno, Martí Aldea).Pages 375-384ģD Tracking of Migrating Cells from Live Microscopy Time-Lapses (Sébastien Tosi, Kyra Campbell).Pages 385-395Ī Cell Segmentation/Tracking Tool Based on Machine Learning (Heather S. HCS Methodology for Helping in Lab Scale Image-Based Assays (Joaquim Soriano, Gadea Mata, Diego Megias).Pages 331-356įilopodia Quantification Using FiloQuant (Guillaume Jacquemet, Hellyeh Hamidi, Johanna Ivaska).Pages 359-373Ĭoincidence Analysis of Molecular Dynamics by Raster Image Correlation Spectroscopy (David F. In Vivo Quantification of Intramolecular FRET Using RacFRET Biosensors (Manel Bosch, Elena Kardash).Pages 275-297Ĭell Proliferation High-Content Screening on Adherent Cell Cultures (Pau Carrillo-Barberà, Jose M. Photobleaching and Sensitized Emission-Based Methods for the Detection of Förster Resonance Energy Transfer (Timo Zimmermann).Pages 235-274 Triple-Colocalization Approach to Assess Traffic Patterns and Their Modulation (Daniel Sastre, Irene Estadella, Manel Bosch, Antonio Felipe).Pages 215-233 Which Elements to Build Co-localization Workflows? From Metrology to Analysis (Patrice Mascalchi, Fabrice P. Structure and Fluorescence Intensity Measurements in Biofilms (Bertrand Cinquin, Filipa Lopes).Pages 117-133ģD + Time Imaging and Image Reconstruction of Pectoral Fin During Zebrafish Embryogenesis (Hanh Nguyen, Jaume Boix-Fabrés, Nadine Peyriéras, Elena Kardash).Pages 135-153Īutomated Macro Approach to Remove Vitelline Membrane Autofluorescence in Drosophila Embryo 4D Movies (Jaume Boix-Fabrés, Katerina Karkali, Enrique Martín-Blanco, Elena Rebollo).Pages 155-175 ![]() ![]() Arbones).Pages 71-97Īutomated Quantitative Analysis of Mitochondrial Morphology (Anna Bosch, Maria Calvo).Pages 99-115 Introduction to ImageJ Macro Language in a Particle Counting Analysis: Automation Matters (Manel Bosch).Pages 51-70Īutomated Macro Approach to Quantify Synapse Density in 2D Confocal Images from Fixed Immunolabeled Neural Tissue Sections (Elena Rebollo, Jaume Boix-Fabrés, Maria L. Proximity Ligation Assay Image Analysis Protocol: Addressing Receptor-Receptor Interactions (Marc López-Cano, Víctor Fernández-Dueñas, Francisco Ciruela).Pages 41-50 Open Source Tools for Biological Image Analysis (Romain Guiet, Olivier Burri, Arne Seitz).Pages 23-37 Main Steps in Image Processing and Quantification: The Analysis Workflow (José F. ![]()
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